Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 12 CD34/PDGFR-α levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT: resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 13 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-α used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 15 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-β used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 14 CD34/PDGFR-β levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control

Journal: Biology of reproduction

Article Title: Prenatal exposure to estrogenic compounds alters the expression pattern of platelet-derived growth factor receptors alpha and beta in neonatal rat testis: identification of gonocytes as targets of estrogen exposure.

doi: 10.1095/biolreprod.102.009605

Figure Lengend Snippet: FIG. 4. Quantification of in situ hybridization signals for PDGFRa and b mRNAs in 3-day-old testis sections. Densitometric analysis was per- formed as described in the Materials and Methods section. The histograms represent the specific intensities of signals, expressed in arbitrary units, for PDGFRa (A) and PDGFRb (B) that were present in the interstitium, periphery, and center of the seminiferous cords of the same samples as Figure 3. *P , 0.05; ***P , 0.001.

Article Snippet: Rabbit polyclonal antibodies against the Cterminal domains of PDGFRa and PDGFRb were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), while rabbit polyclonal antibodies against the kinase domain of PDGFRb was purchased from BD Biosciences (BD Pharmingen, San Diego, CA).

Techniques: In Situ Hybridization

Journal: Cancers

Article Title: Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway

doi: 10.3390/cancers15133375

Figure Lengend Snippet: TAFs promoted PA progression by inhibiting miR-145-5p in PA cells. ( a ) Correlation of PA texture with PA size and aggressiveness (n = 242). ( b ) Immunohistochemical staining of TAFs indicators (α-SMA, PDGFRB, TAGLN) between soft and tough textual tumor (scale bar = 100 μm) (n = 49). ( c – e ) Correlation of TAFs density with PA size and aggressiveness. ( f ) Schematic diagram of extracting NFs and TAFs (figure created with Biorender.com). ( g ) Cell viability experiments of showing effect of TAFs supernatant on indicated PA cells (n = 6), PA1221 and PA0222 were primary PA cells. ( h ) Transwell assay of effect of TAFs supernatant on indicated PA cells (n = 6). ( i ) Analysis of tumor volume and tumor weight after indicated PA cells were mixed with NFs or TAFs (n = 6) (figure was created with Biorender.com). ( j ) Venn diagram of upregulated circRNAs and downregulated miRNAs in PA. ( k ) Heatmap of downregulated miRNAs in PA. ( l ) Correlation analysis of 5 miRNAs with TAF density (n = 15). ( m ) MiR-145-5p expression level after TAFs supernatant effect on PA cells (n = 3). ( n ) Cell viability experiments of effect of TAFs supernatant on PA cells transfected with miR-145-5p mimic (n = 6). ( o ) Colony formation assay of TAFs supernatant effect on PA cells transfected with miR-145-5p mimic (n = 3). ( p ) Analysis of tumor size and weight after miR-145-5p antagomir injection (5 nmol/3 days) (n = 6) (scale bar = 10 mm). The original magnification was ×200. Data were expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Pearson correlation and linear regression were used to compare these parameters between two variables. One-way ANOVA was used for multi-sample comparison and Student’s t -test was used for comparison between two groups. Statistical significance was considered to be indicated by a value of p < 0.05. CM: conditioned medium.

Article Snippet: The tissues were blocked by 5% BSA for 1 h; then, the primary antibodies—α-SMA (Santa Cruz, CA, USA, cat: sc-32251), dilution ratio 1:200; PDGFRB (Proteintech, Wuhan, China), dilution ratio 1:50; TAGLN (Proteintech, Wuhan, China), dilution ratio 1:20, FGFR3 (Proteintech, Wuhan, China, cat: 66954-1-Ig), dilution ratio: 1:200—were applied overnight at 4 °C.

Techniques: Immunohistochemical staining, Staining, Transwell Assay, Expressing, Transfection, Colony Assay, Injection, Comparison

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Reagent information.

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Concentration Assay, Injection

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: MSCs migrated toward ECs via PDGF-BB/PDGFR β in the inflammatory microenvironment. (a) Representative images of migrated hBMSCs that had received different pre-treatments or had been exposed to different inducing media. The migration capacity of hBMSCs was determined using a Transwell culture system. The quantification of migrated cells was shown as a bar graph. Scale bar, 50 μ m. ∗ P < 0.05. (b) Representative images of wound healing assays. The rate of scratch wound closure was shown as a bar graph. Scale bar, 200 μ m. ∗ P < 0.05. EGM: endothelial cell growth medium-2. EC-CM: conditioned media of endothelial cells. IEC-CM: conditioned media of endothelial cells in the context of inflammatory microenvironment; shPDGF-BB: short hairpin RNA targeting pdgfb ; shPDGFR β : shRNA targeting pdgfrb .

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Migration, shRNA

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Src and Akt functioned downstream of PDGFR β . (a) Representative images of migrated hBMSCs in Transwell culture systems. The quantification of migrated cells was shown as a bar graph. Data were compared with the group of IEC-CM from . Scale bar, 50 μ m. ∗ P < 0.05. (b) Representative images of wound healing assays. The rate of scratch wound closure was shown as a bar graph. Data were compared with the group of IEC-CM from . Scale bar, 200 μ m. ∗ P < 0.05. IEC-CM: conditioned media of endothelial cells in the context of inflammatory microenvironment; shPDGFR β : short hairpin RNA targeting pdgfrb ; shSrc: shRNA targeting src ; shAkt: shRNA targeting akt .

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: shRNA

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Src bridged connection between PDGFR β and Akt during ECs-induced MSCs migration. (a) Gene and protein expression of Src in migrating hBMSCs. (b) Gene and protein expression of Akt in migrating hBMSCs. EC-CM: conditioned media of ECs; IEC-CM: conditioned media of ECs in the context of inflammatory microenvironment; shPDGFR β : short hairpin RNA targeting pdgfrb ; shSrc: shRNA targeting src ; shAkt: shRNA targeting akt . ∗ P < 0.05.

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Migration, Expressing, shRNA

Journal: Journal of Translational Medicine

Article Title: Papillary thyroid cancer organoids harboring BRAF V600E mutation reveal potentially beneficial effects of BRAF inhibitor-based combination therapies

doi: 10.1186/s12967-022-03848-z

Figure Lengend Snippet: List of drugs used in this study

Article Snippet: VEGFR, PDGFRβ , Lenvatinib , MedChemExpress , HY-10981 , 10.

Techniques: DNA Synthesis

Journal: Frontiers in pharmacology

Article Title: Extract of Marsdenia tenacissima (Roxb.) Moon [Apocynaceae] Suppresses Hepatocellular Carcinoma by Inhibiting Angiogenesis.

doi: 10.3389/fphar.2022.900128

Figure Lengend Snippet: FIGURE 2 | Identification and verification of differentially expressed genes (DEGs) between HCC cells treated with or without MTE. (A) HepG2 cells were treated with vehicle, 1.5 mg/ml MTE, or 3.0 mg/ml MTE for 24 h and subjected to RNA-sequencing. The clustering of differentially expressed genes of control vs. MTE-1.5 mg/ ml and control vs. MTE-3.0 mg/ml was presented. The DEGs associated with metabolism and angiogenesis were clustered. (B) mRNA expression levels of VWF, VEGFR, and PDGFRB in MHCC97H and HepG2 cells treated with or without MTE. (C) Protein levels of VWF, VEGFR, and PDGFRB in HCC cells treated with or without MTE. *p < 0.05; **p < 0.01.

Article Snippet: After blocking, the membranes were incubated with the primary antibodies against VEGFA (A12303, ABclonal, China), PDGFRB (A2180, ABclonal, China), VWF (A13523, ABclonal, China), and the internal reference β-actin (AC026, ABclonal, China) at 4°C overnight.

Techniques: RNA Sequencing, Control, Expressing

Journal: Frontiers in pharmacology

Article Title: Extract of Marsdenia tenacissima (Roxb.) Moon [Apocynaceae] Suppresses Hepatocellular Carcinoma by Inhibiting Angiogenesis.

doi: 10.3389/fphar.2022.900128

Figure Lengend Snippet: FIGURE 5 | MTE inhibited tumor angiogenesis in the HCC-PDX mouse model. (A) Representative images of IHC of angiogenesis-associated proteins (CD31, PDGFRB, VWF, PDGFB, and VEGF) on pathological slides of HCC-PDX tissues from control, MTE-L, MTE-M, and MTE-H groups. (B) Score of CD31, PDGFB, VEGF, PDGFRB, and VWF positive staining in these slides. Magnification: ×200. Compared with control, *p < 0.05; **p < 0.01; ***p < 0.001. Compared with MTE-L, #p < 0.05; ##p < 0.01; ###p < 0.001.

Article Snippet: After blocking, the membranes were incubated with the primary antibodies against VEGFA (A12303, ABclonal, China), PDGFRB (A2180, ABclonal, China), VWF (A13523, ABclonal, China), and the internal reference β-actin (AC026, ABclonal, China) at 4°C overnight.

Techniques: Control, Staining